PARD6A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-953

Novus Biologicals, part of Bio-Techne
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NB300-953
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PARD6A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GSRIRGDGSGFSL corresponding to C-Terminus according to NP_058644.1, NP_001032358.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PARD6A Antibody

Western Blot: PARD6A Antibody [NB300-953]

Western Blot: PARD6A Antibody [NB300-953]

Western Blot: PARD6A Antibody [NB300-953] - (2ug/ml) staining of Jurkat (A) and U251 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953]

Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953]

Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953] - (10ug/ml) staining of paraffin embedded Human Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Flow Cytometry: PARD6A Antibody [NB300-953]

Flow Cytometry: PARD6A Antibody [NB300-953]

Flow Cytometry: PARD6A Antibody [NB300-953] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for PARD6A Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave no signal but low background in human brain and pancreas extracts at up to 1 ug/ml. IHC-P: Human pancreas show strong cytoplasmic staining in rare cells of intralobular ducts. Human heart shows speckled staining on myocardial fibres in transverse section.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PARD6A

This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

PAR-6, PAR-6 alpha, par-6 partitioning defective 6 homolog alpha (C. elegans), PAR6A, PAR6alpha, PAR-6Apar-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6C, partitioning defective 6 homolog alpha, partitioning defective-6 homolog alpha, partitioning-defective protein 6, Tax interaction protein 40, TAX40, Tax-interacting protein 40, TIP-40PAR6

Entrez Gene IDs

50855 (Human)

Gene Symbol

PARD6A

UniProt

NP_058644. (Human)

Additional PARD6A Products

Product Documents for PARD6A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PARD6A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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