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PARG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89450

Novus Biologicals, part of Bio-Techne
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NBP1-89450
NBP1-89450-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WQRRCTEIVAIDALHFRRYLDQFVPEKMRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAA

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30459355).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARG Antibody

Immunohistochemistry-Paraffin: PARG Antibody [NBP1-89450]

Immunohistochemistry-Paraffin: PARG Antibody [NBP1-89450]

Immunohistochemistry-Paraffin: PARG Antibody [NBP1-89450] - Staining in human testis and pancreas tissues using anti-PARG antibody. Corresponding PARG RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: PARG Antibody [NBP1-89450]

Immunocytochemistry/ Immunofluorescence: PARG Antibody [NBP1-89450]

Immunocytochemistry/Immunofluorescence: PARG Antibody [NBP1-89450] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PARG Antibody [NBP1-89450]

Immunohistochemistry-Paraffin: PARG Antibody [NBP1-89450]

Immunohistochemistry-Paraffin: PARG Antibody [NBP1-89450] - Staining of human pancreas shows low expression as expected.

Applications for PARG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARG

Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq]

Long Name

Poly [ADP-ribose] Glycohydrolase

Alternate Names

PARG99

Gene Symbol

PARG

Additional PARG Products

Product Documents for PARG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PARG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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