PARP Antibody (194C1439) - Cleaved - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27335

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat (Negative)

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 194C1439

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all PARP Antibodies »

Product Specifications

Immunogen

This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.

Specificity

This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PARP Antibody (194C1439) - Cleaved - Azide and BSA Free

Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]

Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]

Western Blot: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, using this antibody. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.
Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved - Azide and BSA Free [NBP2-27335]

Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved - Azide and BSA Free [NBP2-27335]

Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]

Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]

Western Blot: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - Analysis using 293T lysates expressing PARP. Image from verified customer review.

Applications for PARP Antibody (194C1439) - Cleaved - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Western Blot

1-2ug/ml~. Use reported in scientific literature (PMID 27470599)
Application Notes
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-27335 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARP

The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Gene Symbol

PARP1

Additional PARP Products

Product Documents for PARP Antibody (194C1439) - Cleaved - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PARP Antibody (194C1439) - Cleaved - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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