Parvalbumin Antibody (3C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50038

Novus Biologicals, part of Bio-Techne
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NBP2-50038
NBP2-50038SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3C9

Concentration

1 mg/ml

View all Parvalbumin Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Parvalbumin expressed in and purified from E. coli. [UniProt# P20472]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Parvalbumin Antibody (3C9)

Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038]

Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038]

Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse parvalbumin mAb, dilution 1:1,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the parvalbumin antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.
Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]

Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]

Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of rat cerebellum section stained with mouse mAb to parvalbumin, NBP2-50038, dilution 1:1,000, in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear are green.
Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]

Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]

Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Adult rat cerebellum floating section was stained with NBP2-50038 at 1:1,000 (green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue is a DNA stain.

Applications for Parvalbumin Antibody (3C9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:1000 - 1:5000
Application Notes
In WB, this antibody recognizes a band at 12 kDa in skeletal muscle lysates, which represents Parvalbumin. Note, this antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Parvalbumin

Parvalbumin is encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation.

Alternate Names

D22S749, MGC116759, parvalbumin, parvalbumin alpha, PV

Gene Symbol

PVALB

UniProt

P20472

Additional Parvalbumin Products

Product Documents for Parvalbumin Antibody (3C9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Parvalbumin Antibody (3C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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