Parvalbumin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1541
Key Product Details
Species Reactivity
Human, Rat
Applications
Flow Cytometry, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-GVDEFSTLVAES corresponding to C-Terminus according to NP_002845.1.
Epitope
C Terminus
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Parvalbumin Antibody
![Western Blot: Parvalbumin Antibody [NB100-1541]](https://resources.bio-techne.com/images/products/Parvalbumin-Antibody-Western-Blot-NB100-1541-img0002.jpg "Western Blot: Parvalbumin Antibody [NB100-1541]")
Western Blot: Parvalbumin Antibody [NB100-1541]
Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.![Flow Cytometry: Parvalbumin Antibody [NB100-1541]](https://resources.bio-techne.com/images/products/Parvalbumin-Antibody-Flow-Cytometry-NB100-1541-img0001.jpg "Flow Cytometry: Parvalbumin Antibody [NB100-1541]")
Flow Cytometry: Parvalbumin Antibody [NB100-1541]
Flow Cytometry: Parvalbumin Antibody [NB100-1541] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for Parvalbumin Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Peptide ELISA
Detection limit 1:64000
Western Blot
0.01-0.3 ug/ml
Application Notes
Western blot: Approx 15kDa band observed in Human Cerebellum lysates and approx 16kDa in Rat Brain lysates (calculated MW of 12.1kDa according to Human NP_002845.1 and 11.9kDa according to Rat NP_071944.1). These molecular weights are observed by other sources and were successfully blocked by incubation with the immunizing peptide.
Flow Cytometry: Flow cytometric analysis of Kelly cells.
Flow Cytometry: Flow cytometric analysis of Kelly cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Parvalbumin
Alternate Names
D22S749, MGC116759, parvalbumin, parvalbumin alpha, PV
Gene Symbol
PVALB
UniProt
Additional Parvalbumin Products
Product Documents for Parvalbumin Antibody
Product Specific Notices for Parvalbumin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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