Parvalbumin Antibody

Western Blot: Parvalbumin Antibody [NBP2-50036]

Western Blot: Parvalbumin Antibody [NBP2-50036] - Analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken parvalbumin pAb, dilution 1:2,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] recombinant full length human parvalbumin, [4] recombinant full length human calretinin and [5] recombinant full length human calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The parvalbumin antibody binds parvalbumin strongly but is not cross-reactive with the related calcium binding proteins calretinin and calbindin.

Immunohistochemistry Free-Floating: Parvalbumin Antibody [NBP2-50036]

Immunohistochemistry Free-Floating: Parvalbumin Antibody [NBP2-50036] - Analysis of a rat cerebellum section stained with chicken pAb to parvalbumin, NBP2-50036, dilution 1:2,000, in green, and costained with mouse mAb to GFAP, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. NBP2-50036 antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.