Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 1H9

Format

BSA Free

Concentration

0.5 mg/ml

View all Pax5/BSAP Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is Pax5, BSAP (1H9).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Pax5/BSAP Antibody (1H9) - BSA Free

Western Blot: Pax5/BSAP Antibody (1H9)BSA Free [NBP2-00258]

Western Blot: Pax5/BSAP Antibody (1H9)BSA Free [NBP2-00258]

Western Blot: Pax5/BSAP Antibody (1H9) [NBP2-00258] - Daudi cell lysates prepared under reducing conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of Anti-Human/Mouse Pax5 Purified. Bands were visualized using Anti-Rat IgG HRP.
Flow Cytometry: Pax5/BSAP Antibody (1H9) - BSA Free [NBP2-00258]

Flow Cytometry: Pax5/BSAP Antibody (1H9) - BSA Free [NBP2-00258]

Flow Cytometry: Pax5/BSAP Antibody (1H9) [NBP2-00258] - Analysis using the PE conjugate of NBP2-00258. Surface staining of C57BL/6 splenocytes with Anti-Mouse CD23 PE-Cy7 and Anti-Mouse CD21/CD35 FITC followed by intracellular staining using the Foxp3 Staining Buffer Set and 0.125 ug of Anti-Human/Mouse Pax5 PE. Histogram depicts cell populations based on surface stains comprising the marginal zone cells (CD21(high)CD23-) (blue histogram) or T1 cells (CD21-CD23-) (green histogram).

Applications for Pax5/BSAP Antibody (1H9) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This 1H9 antibody has been tested by western blot analysis. This can be used at less than or equal to 5ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This 1H9 antibody has also been reported for use in immunoprecipitation and immunohistology staining of frozen tissue sections.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pax5/BSAP

The monoclonal antibody 1H9 recognizes both mouse and human Pax5. Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (pax) family of transcription factors. Pax5 is the only member of the pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VHDJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Pax5 expression is correlated with many neoplasms. In diffuse large B cell lymphomas (DLBCL) and non-Hodgkin lymphomas Pax5 is often mutated while in B-cell ALL, expression levels are high. Additionally, translocation with elastin, IGH, ETV6, FOXP1, and EVI3 have been identified.

Long Name

Paired Box Gene 5/B-cell Specific Activator Protein

Alternate Names

BSAP

Gene Symbol

PAX5

Additional Pax5/BSAP Products

Product Documents for Pax5/BSAP Antibody (1H9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Pax5/BSAP Antibody (1H9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.