Pax6 Antibody (PAX6/1166) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47915
![Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) - Azide and BSA Free [NBP2-47915]](https://resources.bio-techne.com/images/products/Pax6-Antibody-PAX6-1166-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47915-img0002.jpg "Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) - Azide and BSA Free [NBP2-47915]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PAX6/1166
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant fragment (N-terminus; aa 1-300) of human Pax6 protein (Uniprot: P26367)
Localization
Nuclear
Marker
Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44576).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Pax6 Antibody (PAX6/1166) - Azide and BSA Free
Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) - Azide and BSA Free [NBP2-47915]
Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) - Azide and BSA Free [NBP2-47915] - Human Gastric Carcinoma stained with PAX6 Monoclonal Antibody (PAX6/1166).Applications for Pax6 Antibody (PAX6/1166) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Pax6
Long Name
Paired Box Gene 6
Alternate Names
keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6
Gene Symbol
PAX6
Additional Pax6 Products
Product Documents for Pax6 Antibody (PAX6/1166) - Azide and BSA Free
Product Specific Notices for Pax6 Antibody (PAX6/1166) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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