Pax6 Antibody (PAX6/1166)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44576

Novus Biologicals, part of Bio-Techne
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NBP2-44576-0.1mg
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Flow (Intracellular), Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PAX6/1166

Concentration

0.2 mg/ml

View all Pax6 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (N-terminus; aa 1-300) of human Pax6 protein (Uniprot: P26367)

Localization

Nuclear

Marker

Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47915)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Pax6 Antibody (PAX6/1166)

Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) [NBP2-44576]

Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) [NBP2-44576]

Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) [NBP2-44576] - Human Gastric Carcinoma stained with PAX6 Monoclonal Antibody (PAX6/1166).
Knockout Validated: Pax6 Antibody (PAX6/1166) [NBP2-44576]

Knockout Validated: Pax6 Antibody (PAX6/1166) [NBP2-44576]

Knockout Validated: Pax6 Antibody (PAX6/1166) [NBP2-44576] - Parental and MSN KO cells were labeled with a green or a far-red dye, respectively. Parental and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with anti-Moesin (NBP2-44576) antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining), and far-red (KO) channels was performed. Representative grayscale images of the red channel are shown, and WT and KO cells are outlined with a yellow and magenta dashed line, respectively. A schematic representation of the mosaic strategy used is shown on the right panel. Antibody dilution used: NBP2-44579 at 1/200. Bar = 10 um. Images and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Pax6 Antibody (PAX6/1166)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Pax6

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Gene Symbol

PAX6

UniProt

P26367

Additional Pax6 Products

Product Documents for Pax6 Antibody (PAX6/1166)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pax6 Antibody (PAX6/1166)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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