Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PAX7/1187

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pax7 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)

Localization

Nuclear

Marker

Rhabdomyosarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Pax7 Antibody (PAX7/1187) - IHC-Prediluted

Immunohistochemistry-Paraffin: Pax7 Antibody (PAX7/1187) - IHC-Prediluted [NBP2-48248]

Immunohistochemistry-Paraffin: Pax7 Antibody (PAX7/1187) - IHC-Prediluted [NBP2-48248]

Immunohistochemistry-Paraffin: Pax7 Antibody (PAX7/1187) - IHC-Prediluted [NBP2-48248] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with PAX7 Monoclonal Antibody (PAX7/1187).
Immunohistochemistry-Paraffin: Pax7 Antibody (PAX7/1187) - IHC-Prediluted [NBP2-48248]

Immunohistochemistry-Paraffin: Pax7 Antibody (PAX7/1187) - IHC-Prediluted [NBP2-48248]

Immunohistochemistry-Paraffin: Pax7 Antibody (PAX7/1187) - IHC-Prediluted [NBP2-48248] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with PAX7 Monoclonal Antibody (PAX7/1187).

Applications for Pax7 Antibody (PAX7/1187) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Pax7

PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.

Long Name

Paired Box Gene 7

Alternate Names

HUP1, RMS2

Gene Symbol

PAX7

Additional Pax7 Products

Product Documents for Pax7 Antibody (PAX7/1187) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Pax7 Antibody (PAX7/1187) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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