PAX8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1065

Novus Biologicals, part of Bio-Techne
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NB100-1065
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PAX8 Antibodies »

Product Specifications

Immunogen

Peptide with sequence PHNSIRSGHGGLN-C corresponding to N-Terminus according to NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Specificity

This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAX8 Antibody

Western Blot: PAX8 Antibody [NB100-1065]

Western Blot: PAX8 Antibody [NB100-1065]

Western Blot: PAX8 Antibody [NB100-1065] - Staining of Human Kidney (A) and HeLa cell (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065]

Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065]

Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - Staining of paraffin embedded Human Kidney Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PAX8 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Western Blot

1 - 2 ug/mL
Application Notes
WB: Approx. 45 kDa band observed in human kidney lysates (calculated MW of 43.0 kDa band according to NP_039246.1 and 48.2 kDa band according to NP_003457.1).
IHC-P: A customer has reported unsuccessful use on mouse tissues.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAX8

This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described.

Alternate Names

paired box 8, paired box gene 8, paired box protein Pax-8, paired domain gene 8

Entrez Gene IDs

7849 (Human); 18510 (Mouse); 81819 (Rat)

Gene Symbol

PAX8

UniProt

Q06710 (Human)

Additional PAX8 Products

Product Documents for PAX8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PAX8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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