Paxillin Antibody (S04-3C3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20046

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20046-100ul
NBP3-20046-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-3C3

Concentration

0.3 mg/ml

View all Paxillin Antibodies »

Product Summary for Paxillin Antibody (S04-3C3)

Immunogen

A synthetic peptide of human Paxillin (Uniprot # P49023)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Paxillin Antibody (S04-3C3)

Western Blot: Paxillin Antibody (S04-3C3) [NBP3-20046]

Western Blot: Paxillin Antibody (S04-3C3) [NBP3-20046]

Western Blot: Paxillin Antibody (S04-3C3) [NBP3-20046] - Western blot detection of Paxillin in C6 cell lysates using NBP3-20046 (1:1000 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.
Immunocytochemistry/ Immunofluorescence: Paxillin Antibody (S04-3C3) [NBP3-20046]

Immunocytochemistry/ Immunofluorescence: Paxillin Antibody (S04-3C3) [NBP3-20046]

Immunocytochemistry/Immunofluorescence: Paxillin Antibody (S04-3C3) [NBP3-20046] - Immunofluorescence of Paxillin (green) in hela using NBP3-20046 at dilution 1/5, and DAPI (blue)
Immunohistochemistry-Paraffin: Paxillin Antibody (S04-3C3) [NBP3-20046]

Immunohistochemistry-Paraffin: Paxillin Antibody (S04-3C3) [NBP3-20046]

Immunohistochemistry-Paraffin: Paxillin Antibody (S04-3C3) [NBP3-20046] - Immunohistochemistry of Paxillin in paraffin-embedded Human breast cancer tissue using NBP3-20046 at dilution 1/50

Applications for Paxillin Antibody (S04-3C3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Immunohistochemistry

1/20-1/100

Immunohistochemistry-Paraffin

1:20-1:100

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Paxillin

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Alternate Names

PXN

Gene Symbol

PXN

Additional Paxillin Products

Product Documents for Paxillin Antibody (S04-3C3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Paxillin Antibody (S04-3C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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