PAXX Antibody

Immunohistochemistry-Paraffin: PAXX Antibody [NBP1-94172]

Immunohistochemistry-Paraffin: PAXX Antibody [NBP1-94172] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: PAXX Antibody [NBP1-94172]

Immunohistochemistry-Paraffin: PAXX Antibody [NBP1-94172] - Staining of human prostate shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: PAXX Antibody [NBP1-94172]

Immunohistochemistry-Paraffin: PAXX Antibody [NBP1-94172] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Knockout Validated: PAXX Antibody [NBP1-94172]

Knockout Validated: PAXX Antibody [NBP1-94172] - Generation of Paxx-/- mice. Western blot analyses using Novus anti-PAXX immunoglobulins revealed the lack of PAXX protein in Paxx-/- tail fibroblasts, spleen, thymus, liver, and lungs when compared to Paxx+/+ controls and reduction of PAXX protein in Paxx+/- cells.

Knockout Validated: PAXX Antibody [NBP1-94172]

Knockout Validated: PAXX Antibody [NBP1-94172] - Generation of Paxx-/- mice. Western blot analyses using Novus anti-PAXX immunoglobulins revealed the lack of PAXX protein in Paxx-/- tail fibroblasts, spleen, thymus when compared to Paxx+/+ controls and reduction of PAXX protein in Paxx+/- cells. Image collected and cropped by CiteAb from the following publication (https://febs.onlinelibrary.wiley.com/doi/full/10.1002/2211-5463.12381), licensed under a CC-BY license.

Western Blot: PAXX Antibody [NBP1-94172] -

Western Blot: PAXX Antibody [NBP1-94172] - Generation of Paxx−/− mice. (A) Top. Schematic diagram of murine Paxx locus indicating the ATG & STOP sites, & the targeted loci in the promoter region for sgRNA#1 & in exon 2 for sgRNA#2. Bottom. Resulting Paxx−/− locus lacking part of the promoter region, transcription start site, & exon 1. (B) PCR‐based genotyping strategy reveals the Paxx wt allele (965 bp) & Paxx null allele (270 bp). The following samples are presented, from left to right: two Paxx+/+ & two Paxx−/−. (C,D) Western blot analyses using Novus anti‐PAXX immunoglobulins revealed the lack of PAXX protein in Paxx−/− tail fibroblasts, spleen, thymus, liver, & lungs when compared to Paxx+/+ controls & reduction of PAXX protein in Paxx+/− cells. (E) Western blot using Abcam anti‐PAXX immunoglobulins showed no PAXX protein in Paxx−/− & reduction of PAXX protein in Paxx+/− livers compared to Paxx+/+. (F) Analyses of 184 pups born from Paxx+/− parents revealed expected genetic distribution of Paxx+/+ (43), Paxx+/− (89), & Paxx−/− (52) mice, which is close to 1 : 2 : 1 distribution. (G) Body weight of 30‐day‐old Paxx+/+ mice (n = 43) is not distinguishable from Paxx+/− (n = 60, P = 0.9559) & Paxx−/− mice (n = 99, P = 0.9629). Six Ku80−/− mice of the same age were significantly smaller than Paxx+/+, Paxx+/−, & Paxx−/− mice, P < 0.0001. (H) Example of 5‐week‐old mice, Paxx+/+, two Paxx+/−, & Paxx−/−, as indicated. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29511619), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PAXX Antibody [NBP1-94172] -

Western Blot: PAXX Antibody [NBP1-94172] - Generation of Paxx−/− mice. (A) Top. Schematic diagram of murine Paxx locus indicating the ATG & STOP sites, & the targeted loci in the promoter region for sgRNA#1 & in exon 2 for sgRNA#2. Bottom. Resulting Paxx−/− locus lacking part of the promoter region, transcription start site, & exon 1. (B) PCR‐based genotyping strategy reveals the Paxx wt allele (965 bp) & Paxx null allele (270 bp). The following samples are presented, from left to right: two Paxx+/+ & two Paxx−/−. (C,D) Western blot analyses using Novus anti‐PAXX immunoglobulins revealed the lack of PAXX protein in Paxx−/− tail fibroblasts, spleen, thymus, liver, & lungs when compared to Paxx+/+ controls & reduction of PAXX protein in Paxx+/− cells. (E) Western blot using Abcam anti‐PAXX immunoglobulins showed no PAXX protein in Paxx−/− & reduction of PAXX protein in Paxx+/− livers compared to Paxx+/+. (F) Analyses of 184 pups born from Paxx+/− parents revealed expected genetic distribution of Paxx+/+ (43), Paxx+/− (89), & Paxx−/− (52) mice, which is close to 1 : 2 : 1 distribution. (G) Body weight of 30‐day‐old Paxx+/+ mice (n = 43) is not distinguishable from Paxx+/− (n = 60, P = 0.9559) & Paxx−/− mice (n = 99, P = 0.9629). Six Ku80−/− mice of the same age were significantly smaller than Paxx+/+, Paxx+/−, & Paxx−/− mice, P < 0.0001. (H) Example of 5‐week‐old mice, Paxx+/+, two Paxx+/−, & Paxx−/−, as indicated. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29511619), licensed under a CC-BY license. Not internally tested by Novus Biologicals.