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PCGF1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16635

Novus Biologicals, part of Bio-Techne
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NBP3-16635-100ul
NBP3-16635-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for PCGF1 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 160-259 of human PCGF1 (NP_116062.2). AHYYRYDEQLNLCLERLSSGKDKNKSVLQNKYVRCSVRAEVRHLRRVLCHRLMLNPQHVQLLFDNEVLPDHMTMKQIWLSRWFGKPSPLLLQYSVKEKRR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCGF1 Antibody - Azide and BSA Free

Western Blot: PCGF1 AntibodyAzide and BSA Free [NBP3-16635]

Western Blot: PCGF1 AntibodyAzide and BSA Free [NBP3-16635]

Western Blot: PCGF1 Antibody [NBP3-16635] - Western blot analysis of extracts of various cell lines, using PCGF1 antibody (NBP3-16635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: PCGF1 Antibody - Azide and BSA Free [NBP3-16635]

Immunocytochemistry/ Immunofluorescence: PCGF1 Antibody - Azide and BSA Free [NBP3-16635]

Immunocytochemistry/Immunofluorescence: PCGF1 Antibody [NBP3-16635] - Immunofluorescence analysis of U-2OS cells using PCGF1 antibody (NBP3-16635) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for PCGF1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PCGF1

PCGF1 is a mammalian homolog of the Drosophila polycomb group genes, which act as transcriptional repressors toregulate anterior-posterior patterning in early embryonic development (Nunes et al., 2001 (PubMed 11287196)). See alsoPCGF2 (MIM 600346).(supplied by OMIM)

Alternate Names

MGC10882, Nervous system Polycomb-1, NSPc1, NSPC1FLJ43754, polycomb group ring finger 1,2010002K04Rik, polycomb group RING finger protein 1, RING finger protein 68, RNF68RNF3A-2

Gene Symbol

PCGF1

Additional PCGF1 Products

Product Documents for PCGF1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCGF1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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