PCNA Antibody (339)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-478

Novus Biologicals, part of Bio-Techne
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NB100-478
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 339

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCNA Antibodies »

Product Specifications

Immunogen

Recombinant PCNA interdomain connector loop protein

Localization

Nuclear

Specificity

This reacts with the interdomain connector loop of human PCNA, other not determined.

Marker

Proliferation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PCNA Antibody (339)

Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (339) [NB100-478]

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (339) [NB100-478]

Immunocytochemistry/Immunofluorescence: PCNA Antibody (339) [NB100-478] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using 1:500 dilution.
Western Blot: PCNA Antibody (339) [NB100-478]
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Applications for PCNA Antibody (339)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:500-1:1000
Application Notes
WB: Predicted MW is 31.32 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Entrez Gene IDs

5111 (Human)

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (339)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PCNA Antibody (339)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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