PCNA Antibody (BLR075G)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78712

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR075G

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PCNA Antibodies »

Product Summary for PCNA Antibody (BLR075G)

Immunogen

A peptide representing a region between residue 225 and the C-terminus (residue 261).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNA Antibody (BLR075G)

Western Blot: PCNA Antibody (BLR075G) [NBP2-78712]

Western Blot: PCNA Antibody (BLR075G) [NBP2-78712]

Western Blot: PCNA Antibody (BLR075G) [NBP2-78712] - Detection of human PCNA by WB of A-549, Jurkat, HEK293T, RKO, K-562, MCF-7, HeLa, U2OS, TCMK-1, and NIH 3T3 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).
Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712]

Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712]

Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Antibody: Rabbit anti-PCNA recombinant monoclonal antibody [BLR075G]Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain:DAPI.
Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712]

Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712]

Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Human ovarian carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.
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Applications for PCNA Antibody (BLR075G)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:1000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. IHC and ICC/IF: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: PCNA

Proliferating Cell Nuclear Antigen (PCNA) is a DNA polymerase that is essential for DNA replication, excision and mismatch repair pathways. PCNA is a "DNA clamp" which binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA antibodies are also useful tools for DNA repair and cancer research.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (BLR075G)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PCNA Antibody (BLR075G)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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