PCNA Antibody (PCNA/8303R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23683
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # PCNA/8303R
Concentration
0.2 mg/ml
Product Summary for PCNA Antibody (PCNA/8303R)
Immunogen
Recombinant full-length human PCNA protein
Localization
Nucleus. Cytoplasm.
Marker
G1- & S-phase Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for PCNA Antibody (PCNA/8303R)
Western Blot: PCNA Antibody (PCNA/8303R) [NBP3-23683] -
Western blot analysis of Raji cell lysate using PCNA antibody (PCNA/8303R).
Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/8303R) [NBP3-23683] -
Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (PCNA/8303R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for PCNA Antibody (PCNA/8303R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Western Blot
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbol
PCNA
Additional PCNA Products
Product Documents for PCNA Antibody (PCNA/8303R)
Product Specific Notices for PCNA Antibody (PCNA/8303R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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