PCNA Antibody (rPCNA/8858) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24069
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # rPCNA/8858
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human PCNA protein
Localization
Nucleus. Cytoplasm.
Marker
G1- & S-phase Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for PCNA Antibody (rPCNA/8858) - Azide and BSA Free
Immunohistochemistry-Paraffin: PCNA Antibody (rPCNA/8858) - Azide and BSA Free [NBP3-24069] -
Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (rPCNA/8858) - Azide and BSA Free at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: PCNA Antibody (rPCNA/8858) - Azide and BSA Free [NBP3-24069] -
Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (rPCNA/8858) - Azide and BSA Free at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for PCNA Antibody (rPCNA/8858) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbol
PCNA
Additional PCNA Products
Product Documents for PCNA Antibody (rPCNA/8858) - Azide and BSA Free
Product Specific Notices for PCNA Antibody (rPCNA/8858) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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