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PCNA Antibody (rPCNA/8859)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23682

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-23682-100ug
NBP3-23682-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rPCNA/8859

Concentration

0.2 mg/ml

Product Summary for PCNA Antibody (rPCNA/8859)

Immunogen

Recombinant full-length human PCNA protein

Localization

Nucleus. Cytoplasm.

Marker

G1- & S-phase Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for PCNA Antibody (rPCNA/8859)

PCNA Antibody (rPCNA/8859)

Immunohistochemistry-Paraffin: PCNA Antibody (rPCNA/8859) [NBP3-23682] -

Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (rPCNA/8859) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Applications for PCNA Antibody (rPCNA/8859)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PCNA

Proliferating Cell Nuclear Antigen (PCNA) is a DNA polymerase that is essential for DNA replication, excision and mismatch repair pathways. PCNA is a "DNA clamp" which binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA antibodies are also useful tools for DNA repair and cancer research.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (rPCNA/8859)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCNA Antibody (rPCNA/8859)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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