PCNA [p Tyr211] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-59861
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.05 mg/ml
Product Specifications
Immunogen
Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human proliferating cell nuclear antigen (GeneID 5111) around tyrosine 211 according to the numbering given inentry NP_002583.1.
Modification
p Tyr211
Marker
Proliferation Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PCNA [p Tyr211] Antibody
![Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]](https://resources.bio-techne.com/images/products/PCNA-[p-Tyr211]-Antibody-Immunohistochemistry-NB100-59861-img0005.jpg "Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]")
Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]
Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB![Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]](https://resources.bio-techne.com/images/products/PCNA-[p-Tyr211]-Antibody-Immunohistochemistry-NB100-59861-img0004.jpg "Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]")
Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]
Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used a dilution of 1:250 and incubated for 18 hours at room temeprature. Detection: DABApplications for PCNA [p Tyr211] Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Application Notes
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Extended incubation with primary antibody (16-24 hours) at room temperature is recommended. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.05 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Entrez Gene IDs
5111 (Human)
Gene Symbol
PCNA
UniProt
Additional PCNA Products
Product Documents for PCNA [p Tyr211] Antibody
Product Specific Notices for PCNA [p Tyr211] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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