Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # J43

Format

BSA Free

Concentration

0.5 mg/ml

View all PD-1 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was PD1.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for PD-1 Antibody (J43) - BSA Free

Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]

Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]

Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis of PE conjugate of NBP1-43110. The J43 antibody has been tested by flow cytometric mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be de
Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]

Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]

Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig
Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]

Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]

Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Biotin conjugate of NBP1-43110. Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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Applications for PD-1 Antibody (J43) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.IHC-F reactivity reported in scientific literature (PMID: 12847137 )
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PD-1

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Gene Symbol

PDCD1

Additional PD-1 Products

Product Documents for PD-1 Antibody (J43) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PD-1 Antibody (J43) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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