Key Product Details

Species Reactivity

Validated:

Mouse

Applications

CyTOF-ready, Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # J43

Format

Azide and BSA Free

Concentration

1 mg/ml

View all PD-1 Antibodies »

Product Summary for PD-1 Antibody (J43) - Azide and BSA Free

Immunogen

The immunogen for this antibody was PD1.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for PD-1 Antibody (J43) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (J43) - Azide and BSA Free [NBP2-34497]

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (J43) - Azide and BSA Free [NBP2-34497]

Immunocytochemistry/Immunofluorescence: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.
Flow Cytometry: PD-1 Antibody (J43) - Azide and BSA Free [NBP2-34497]

Flow Cytometry: PD-1 Antibody (J43) - Azide and BSA Free [NBP2-34497]

Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig
Flow Cytometry: PD-1 Antibody (J43) - Azide and BSA Free [NBP2-34497]

Flow Cytometry: PD-1 Antibody (J43) - Azide and BSA Free [NBP2-34497]

Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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Applications for PD-1 Antibody (J43) - Azide and BSA Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cells and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PD-1

PD1 is an inhibitory cell membrane surface receptor from the immunoglobulin superfamily. Involved in the regulation of T cell function during immunity and tolerance, PD1 inhibits T cell effector functions upon ligand binding. The protein is expressed in pro-B-cells and is thought to play a role in their differentiation as well. Studies have also suggested that this gene product may also contribute to the prevention of autoimmune diseases.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Gene Symbol

PDCD1

Additional PD-1 Products

Product Documents for PD-1 Antibody (J43) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PD-1 Antibody (J43) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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