Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PD-1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1. The immunogen is located within amino acids 120 - 170 of PD-1. Amino Acid Squence: LRAELRVTERRAEVPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PD-1 Antibody - BSA Free

Western Blot: PD-1 AntibodyBSA Free [NBP1-77277]

Western Blot: PD-1 AntibodyBSA Free [NBP1-77277]

Western Blot: PD-1 Antibody [NBP1-77277] - THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
PD-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody - BSA Free [NBP1-77277] -

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody - BSA Free [NBP1-77277] - Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 ug/ml.
PD-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody - BSA Free [NBP1-77277] -

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody - BSA Free [NBP1-77277] - Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 ug/ml.

Green: PD-1 Antibody
Blue: DAPI staining
View 1 more images

Applications for PD-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PD-1

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Gene Symbol

PDCD1

UniProt

Q15116

Additional PD-1 Products

Product Documents for PD-1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PD-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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