PD-ECGF/Thymidine Phosphorylase Antibody (SPM322) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54403
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM322
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary
Immunogen
Recombinant full-length human PD-ECGF/Thymidine Phosphorylase protein (Uniprot: P19971)
Localization
Cytoplasmic and Nuclear
Specificity
Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
Marker
Angiogenesis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53396).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Western Blot: PD-ECGF/Thymidine Phosphorylase Antibody (SPM322)Azide and BSA Free [NBP2-54403]
Western Blot: PD-ECGF/Thymidine Phosphorylase Antibody (SPM322) - Azide and BSA Free [NBP2-54403] - Western Blot Analysis of human spleen tissue lysate using PD-ECGF/Thymidine Phosphorylase Monoclonal Antibody (SPM322).Immunohistochemistry-Paraffin: PD-ECGF/Thymidine Phosphorylase Antibody (SPM322) - Azide and BSA Free [NBP2-54403]
Immunohistochemistry-Paraffin: PD-ECGF/Thymidine Phosphorylase Antibody (SPM322) - Azide and BSA Free [NBP2-54403] - Formalin-fixed, paraffin-embedded human Placenta stained with Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (SPM322).Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Immunoprecipitation
0.5 - 1 ug
Western Blot
0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: PD-ECGF/Thymidine Phosphorylase
Long Name
Platelet-derived Endothelial Cell Growth Factor
Alternate Names
dThdPase, ECGF1, Gliostatin, MEDPS1, MNGIE, MTDPS1, PDECGF, TdRPase, Thymidine Phosphorylase, TPase, TYMP
Gene Symbol
TYMP
Additional PD-ECGF/Thymidine Phosphorylase Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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