PDCD4 Antibody (4C2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18224
![Western Blot: PDCD4 Antibody (4C2) [NBP3-18224]](https://resources.bio-techne.com/images/products/PDCD4-Antibody-4C2-Western-Blot-NBP3-18224-img0003.jpg "Western Blot: PDCD4 Antibody (4C2) [NBP3-18224]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4C2
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human PDCD4 (1-469aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for PDCD4 Antibody (4C2)
Western Blot: PDCD4 Antibody (4C2) [NBP3-18224]
Western Blot: PDCD4 Antibody (4C2) [NBP3-18224] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-18224 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate![Immunocytochemistry/ Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224]](https://resources.bio-techne.com/images/products/PDCD4-Antibody-4C2-Immunocytochemistry-Immunofluorescence-NBP3-18224-img0002.jpg "Immunocytochemistry/ Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224]")
Immunocytochemistry/ Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224]
Immunocytochemistry/Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224] - ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with NBP3-18224 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).![Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224]](https://resources.bio-techne.com/images/products/PDCD4-Antibody-4C2-Flow-Cytometry-NBP3-18224-img0001.jpg "Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224]")
Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224]
Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224] - Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with NBP3-18224 at 2-5ug for 1x10^6cells (red). A Goat anti-mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Applications for PDCD4 Antibody (4C2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate-Buffered Saline (pH 7.4), 10% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PDCD4
Long Name
Programmed Cell Death 4
Alternate Names
H731, Protein 197/15a
Gene Symbol
PDCD4
Additional PDCD4 Products
Product Specific Notices for PDCD4 Antibody (4C2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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