PDCD4 Antibody (k4C1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04329

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # k4C1

Format

BSA Free

Concentration

1.0 mg/ml

View all PDCD4 Antibodies »

Product Specifications

Immunogen

Recombinant human PDCD4 (1-469aa) purified from E. coli (NP_055271).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDCD4 Antibody (k4C1) - BSA Free

Western Blot: PDCD4 Antibody (k4C1)BSA Free [NBP1-04329]

Western Blot: PDCD4 Antibody (k4C1)BSA Free [NBP1-04329]

Western Blot: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04329 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate.
Immunocytochemistry/ Immunofluorescence: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329]

Immunocytochemistry/ Immunofluorescence: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329]

Immunocytochemistry/Immunofluorescence: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with NBP1-04329 (1:100). The secondary antibody (green) used was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329]

Flow Cytometry: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329]

Flow Cytometry: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with NBP1-04329 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for PDCD4 Antibody (k4C1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDCD4

Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells.

Long Name

Programmed Cell Death 4

Alternate Names

H731, Protein 197/15a

Entrez Gene IDs

27250 (Human)

Gene Symbol

PDCD4

UniProt

Q53EL6 (Human)

Additional PDCD4 Products

Product Documents for PDCD4 Antibody (k4C1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDCD4 Antibody (k4C1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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