PDCD4 [p Ser457] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB110-60014
![PDCD4 [p Ser457] Antibody](https://resources.bio-techne.com/images/products/nb110-60014_rabbit-polyclonal-pdcd4-p-ser457-antibody-2552023131054.jpg "PDCD4 [p Ser457] Antibody")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified PDCD4 [p Ser457] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human PDCD4 protein. (Uniprot: Q53EL6)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with PDCD4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with PDCD4 from other sources has not been determined.
Modification
p Ser457
Specificity
This antibody is specific for human PDCD4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with PDCD4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with PDCD4 from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for PDCD4 [p Ser457] Antibody
![Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014]](https://resources.bio-techne.com/images/products/PDCD4-[p-Ser457]-Antibody-Western-Blot-NB110-60014-img0007.jpg "Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014]")
Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014]
Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014] - Analysis of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteasome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4).![Immunohistochemistry: PDCD4 [p Ser457] Antibody [NB110-60014]](https://resources.bio-techne.com/images/products/PDCD4-[p-Ser457]-Antibody-Immunohistochemistry-NB110-60014-img0004.jpg "Immunohistochemistry: PDCD4 [p Ser457] Antibody [NB110-60014]")
Immunohistochemistry: PDCD4 [p Ser457] Antibody [NB110-60014]
Immunohistochemistry: PDCD4 [p Ser457] Antibody [NB110-60014] - Used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.![PDCD4 [p Ser457] Antibody](https://resources.bio-techne.com/images/products/nb110-60014_rabbit-polyclonal-pdcd4-p-ser457-antibody-23520231814310.jpg "PDCD4 [p Ser457] Antibody")
PDCD4 [p Ser457] Antibody
affinity purified anti-Pdcd4 pS457 antibody was used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.Applications for PDCD4 [p Ser457] Antibody
Application
Recommended Usage
ELISA
1:400000
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
21.25-2.5 ug/ml
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 52 kDa in size corresponding to Pdcd4 protein is expected in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: PDCD4
Long Name
Programmed Cell Death 4
Alternate Names
H731, Protein 197/15a
Gene Symbol
PDCD4
UniProt
Additional PDCD4 Products
Product Documents for PDCD4 [p Ser457] Antibody
Product Specific Notices for PDCD4 [p Ser457] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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