Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1C9

Concentration

1 mg/ml

View all PDE10A Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human PDE10A expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens phosphodiesterase 10A (PDE10A), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

88.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE10A Antibody (OTI1C9)

Western Blot: PDE10A Antibody (OTI1C9) [NBP1-47909]

Western Blot: PDE10A Antibody (OTI1C9) [NBP1-47909]

Western Blot: PDE10A Antibody (1C9) [NBP1-47909] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A.
Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] Staining of paraffin-embedded prostate tissue using anti-PDE10A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE10A Antibody (OTI1C9) [NBP1-47909]

Immunohistochemistry-Paraffin: PDE10A Antibody (OTI1C9) [NBP1-47909]

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PDE10Amouse monoclonal antibody.
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Applications for PDE10A Antibody (OTI1C9)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE10A

Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the hydrolysis of 3'cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by controlling the rate of their degradation. There are 11 subtypes of PDEs, named PDE1-11; PDE4, 7 and 8 selectively degrade cAMP, PDE5, 6 and 9 selectively degrade cGMP and PDE1, 2, 3, 10 and 11 degrade both cyclic nucleotides. PDEs are expressed ubiquitously,with each subtype having a specific tissue distribution. These enzymes are involved in many signal transduction pathways and their functions include vascular smooth muscle proliferation and contraction, cardiac contractility,platelet aggregation, hormone secretion, immune cell activation, and they are involved in learning and memory.

Long Name

cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A

Alternate Names

EC 3.1.4.17

Entrez Gene IDs

10846 (Human)

Gene Symbol

PDE10A

Additional PDE10A Products

Product Documents for PDE10A Antibody (OTI1C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDE10A Antibody (OTI1C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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