PDE10A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-645
![Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]](https://resources.bio-techne.com/images/products/PDE10A-Antibody-Immunohistochemistry-Paraffin-NB300-645-img0003.jpg "Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Human, Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.7 mg/ml
Product Specifications
Immunogen
Synthetic peptide taken within amino acid region 300-350 on human PDE10A2 protein common to all PDE10A variants.
Localization
Located mostly to soluble cellular fractions.
Specificity
This has no cross-reactivity against any other PDE family members tested so far.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PDE10A Antibody
![Western Blot: PDE10A Antibody [NB300-645]](https://resources.bio-techne.com/images/products/PDE10A-Antibody-Western-Blot-NB300-645-img0005.jpg "Western Blot: PDE10A Antibody [NB300-645]")
Western Blot: PDE10A Antibody [NB300-645]
Western Blot: PDE10A Antibody [NB300-645] - Rat Striatum: 1. Pan-PDE10A antibody. 2. PDE10A2 variant selective antibody. Diluted 1:5000in DiluOBuffer.![Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]](https://resources.bio-techne.com/images/products/PDE10A-Antibody-Immunohistochemistry-Paraffin-img0006.jpg "Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]")
Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]
Immunohistochemistry-Paraffin: PDE10A Antibody - 1:1000 dilution in IHC Block Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X mangnification.FFPE section. Mouse Brain.![Western Blot: PDE10A Antibody [NB300-645]](https://resources.bio-techne.com/images/products/PDE10A-Antibody-Western-Blot-NB300-645-img0001.jpg "Western Blot: PDE10A Antibody [NB300-645]")
Western Blot: PDE10A Antibody [NB300-645]
Western Blot: PDE10A Antibody [NB300-645] - NB300-645 (1:500) using CNS Blot. 1. Frontal Cortex 2. Pariental Cortex 3. Striatum 4. Amygdela 5. Hypothalamus 6. Hippocampus 7. Olfactory bulb 8. Brain Stem 9. Cerebellum 10. Spinal cord and 11. Whole Brain.Applications for PDE10A Antibody
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
> 1:250
Western Blot
> 1:500
Application Notes
ICC/IF reactivity reported in (PMID: 21559457). Detects a band of approximately 90-93 kDa in rat brain and rat pancreas samples. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
Preservative
0.02% Sodium Azide
Concentration
0.7 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PDE10A
Long Name
cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
Alternate Names
EC 3.1.4.17
Gene Symbol
PDE10A
UniProt
Additional PDE10A Products
Product Documents for PDE10A Antibody
Product Specific Notices for PDE10A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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