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PDE3A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85645

Novus Biologicals, part of Bio-Techne
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NBP1-85645
NBP1-85645-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE3A Antibody

Western Blot: PDE3A Antibody [NBP1-85645]

Western Blot: PDE3A Antibody [NBP1-85645]

Western Blot: PDE3A Antibody [NBP1-85645] - Analysis in human cell lines HeLa and HEK293 using Anti-PDE3A antibody. Corresponding PDE3A RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: PDE3A Antibody [NBP1-85645]

Immunocytochemistry/ Immunofluorescence: PDE3A Antibody [NBP1-85645]

Immunocytochemistry/Immunofluorescence: PDE3A Antibody [NBP1-85645] - Immunofluorescent staining of human cell line HeLa shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDE3A Antibody [NBP1-85645]

Immunohistochemistry-Paraffin: PDE3A Antibody [NBP1-85645]

Immunohistochemistry-Paraffin: PDE3A Antibody [NBP1-85645] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Applications for PDE3A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE3A

Hydrolyzes both cyclic AMP (cAMP) and cyclic GMP (cGMP).

Long Name

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Alternate Names

cGI-PDE, CGI-PDE A

Gene Symbol

PDE3A

Additional PDE3A Products

Product Documents for PDE3A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDE3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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