PDE4B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12243

Novus Biologicals, part of Bio-Techne
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NBP3-12243
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PDE4B Antibodies »

Product Summary for PDE4B Antibody

Immunogen

Synthetic peptide common to all PDE4B subtypes taken near N-terminal

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for PDE4B Antibody

Western Blot: PDE4B Antibody [NBP3-12243]

Western Blot: PDE4B Antibody [NBP3-12243]

Western Blot: PDE4B Antibody [NBP3-12243] - wb of NBP3-12243. 1:500 antibody dilution in DiluObuffer.
Immunohistochemistry-Paraffin: PDE4B Antibody [NBP3-12243]

Immunohistochemistry-Paraffin: PDE4B Antibody [NBP3-12243]

Immunohistochemistry-Paraffin: PDE4B Antibody [NBP3-12243] - IHC of NBP3-12243 and human neuroblastoma cells. 1:250 antibody dilution.
Immunohistochemistry-Paraffin: PDE4B Antibody [NBP3-12243]

Immunohistochemistry-Paraffin: PDE4B Antibody [NBP3-12243]

Immunohistochemistry-Paraffin: PDE4B Antibody [NBP3-12243] - Scanning laser microscopy of fixed frozen section of seminiferous tubule stained. Varients are localized in somatic cells, primarily sertoli cells.
View 1 more images

Applications for PDE4B Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:350

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE4B

PDE4B is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. Four genes (PDE4A, B, C and D) make up the PDE4 family. cAMP-dependent phosphodiesterase type B (PDE4B) family consists of four known splice variants, PDE4B1, B2, B3 and a novel 66 kDa PDE4B4 protein. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In CNS, all four PDE4B subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Some PDE4B variants are also substrate for PKA-dependent phosphorylation. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants; the PDE4B4 (66kDa protein) is the major PDE4B variant expressed in lungs, testis and heart. In testis PDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4A is localized in germ cells.

Long Name

cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Alternate Names

DPDE4, PDE32

Gene Symbol

PDE4B

Additional PDE4B Products

Product Documents for PDE4B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDE4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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