PDGF-B Antibody (MOR-8457) - Humanized

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28115

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # MOR-8457

Concentration

LYOPH mg/ml

View all PDGF-B Antibodies »

Product Specifications

Immunogen

PDGFB

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hulambda. It has a predicted MW of 145.5 kDa.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PDGF-B Antibody (MOR-8457) - Humanized

PDGF-B Antibody (MOR-8457) - Humanized

ELISA: PDGF-B Antibody (MOR-8457) - Humanized[NBP3-28115] -

Immobilized Cynomolgus / Rhesus Macaque PDGFB, Fc Tag at 2 μg/mL can bind PDGF-B Antibody (MOR-8457) - Humanized, The EC50 was approximately 0.06539 ug/ml.
PDGF-B Antibody (MOR-8457) - Humanized

ELISA: PDGF-B Antibody (MOR-8457) - Humanized[NBP3-28115] -

Immobilized Mouse PDGFB, His Tag at 2 μg/mL can bind PDGF-B Antibody (MOR-8457) - Humanized, The EC50 was approximately 0.008642 ug/ml.
PDGF-B Antibody (MOR-8457) - Humanized

ELISA: PDGF-B Antibody (MOR-8457) - Humanized[NBP3-28115] -

Immobilized Human PDGFB, Fc Tag at 2 μg/mL can bind PDGF-B Antibody (MOR-8457) - Humanized, The EC50 was approximately 0.07279 ug/ml.

Applications for PDGF-B Antibody (MOR-8457) - Humanized

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PDGF-B

Platelet-Derived Growth Factor-BB (PDGF-BB) is a disulfide-linked homodimer of two B-chains (109 aa each). It is stored in platelet granules and is released folllowing activation of platelets. PDGF-BB has potent mitogenic effects on various cell types such as cells of connective tissue, bone and cartilage and smooth muscle. It promotes bone formation and soft tissue repair, stimulating wound healing processes. PDGF-BB is involved in the development of the nervous system and may act as a neuronal regulatory agent via nerve regeneration and proliferation. It also plays a role in the epithelial cell development of the respiratory system, cell migration and angiogenesis. Benefits of NovActive Proteins, Fully Active, Fully folded naturally by eukaryotic source. Comparable for eukaryotic glycosylation BioRisk Free, animal-free, serum-free, endotoxin-free, infection reagent-free, low in proteolyic activity, low in pyrogenic and pro-inflammatory activity. Just reconstitute and add to your cell culture. Have FDA G.R.A.S. (generally recognized as safe) approval. Advantages of barley endosperm, Folding, Proficient protein machinery, with eukaryotic folding, allows for native formation of the protein and long term protection and storage. Environment, A biochemically inert environment, void of endotoxins, low protease activity and secondary metabolite content, and a simple protein profile, aid in downstream processing in barley. FDA G.R.A.S status. Barley is recognized by the FDA as having G.R.A.S (generally recognized as safe) status.

Long Name

Platelet-derived Growth Factor B

Alternate Names

C-Sis, IBGC5, PDGF-2, PDGF2, PDGFB, SIS, SSV

Gene Symbol

PDGFB

UniProt

P01127

Additional PDGF-B Products

Product Documents for PDGF-B Antibody (MOR-8457) - Humanized

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDGF-B Antibody (MOR-8457) - Humanized

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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