PDGF-B Antibody (PDGFB/3072)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07811

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # PDGFB/3072

Concentration

0.2 mg/ml

View all PDGF-B Antibodies »

Product Summary for PDGF-B Antibody (PDGFB/3072)

Immunogen

Recombinant fragment of human PDGF-B protein (around aa 27-158) (exact sequence is proprietary) (Uniprot: P01127)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08823)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PDGF-B Antibody (PDGFB/3072)

Immunohistochemistry-Paraffin: PDGF-B Antibody (PDGFB/3072) [NBP3-07811]

Immunohistochemistry-Paraffin: PDGF-B Antibody (PDGFB/3072) [NBP3-07811]

Immunohistochemistry-Paraffin: PDGF-B Antibody (PDGFB/3072) [NBP3-07811] - Formalin-fixed, paraffin-embedded human colon stained with PDGF-B Mouse Monoclonal Antibody (PDGFB/3072). Inset: PBS instead of primary antibody, secondary antibody negative control.
Protein Array: PDGF-B Antibody (PDGFB/3072) [NBP3-07811]

Protein Array: PDGF-B Antibody (PDGFB/3072) [NBP3-07811]

Protein Array: PDGF-B Antibody (PDGFB/3072) [NBP3-07811] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PDGF-B Mouse Monoclonal Antibody (PDGFB/3072).

Applications for PDGF-B Antibody (PDGFB/3072)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PDGF-B

Platelet-Derived Growth Factor-BB (PDGF-BB) is a disulfide-linked homodimer of two B-chains (109 aa each). It is stored in platelet granules and is released folllowing activation of platelets. PDGF-BB has potent mitogenic effects on various cell types such as cells of connective tissue, bone and cartilage and smooth muscle. It promotes bone formation and soft tissue repair, stimulating wound healing processes. PDGF-BB is involved in the development of the nervous system and may act as a neuronal regulatory agent via nerve regeneration and proliferation. It also plays a role in the epithelial cell development of the respiratory system, cell migration and angiogenesis. Benefits of NovActive Proteins, Fully Active, Fully folded naturally by eukaryotic source. Comparable for eukaryotic glycosylation BioRisk Free, animal-free, serum-free, endotoxin-free, infection reagent-free, low in proteolyic activity, low in pyrogenic and pro-inflammatory activity. Just reconstitute and add to your cell culture. Have FDA G.R.A.S. (generally recognized as safe) approval. Advantages of barley endosperm, Folding, Proficient protein machinery, with eukaryotic folding, allows for native formation of the protein and long term protection and storage. Environment, A biochemically inert environment, void of endotoxins, low protease activity and secondary metabolite content, and a simple protein profile, aid in downstream processing in barley. FDA G.R.A.S status. Barley is recognized by the FDA as having G.R.A.S (generally recognized as safe) status.

Long Name

Platelet-derived Growth Factor B

Alternate Names

C-Sis, IBGC5, PDGF-2, PDGF2, PDGFB, SIS, SSV

Gene Symbol

PDGFB

UniProt

P01127

Additional PDGF-B Products

Product Documents for PDGF-B Antibody (PDGFB/3072)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDGF-B Antibody (PDGFB/3072)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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