PDGF-B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52533
Key Product Details
Species Reactivity
Validated:
Human, Bovine, Canine, Sheep
Cited:
Mouse, Canine
Predicted:
Bat (92%), Equine (92%), Monkey (100%), Mouse (92%), Opossum (100%), Primate (92%), Rat (92%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Cited:
Block/Neutralize, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KHRKFKHTHDKT, from the C Terminus of the PDGF-B protein sequence according to NP_002599.1 and NP_148937.1.
Epitope
C-Terminus
Reactivity Notes
Canine reactivity reported in scientific literature (PMID: 28883003).Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (92%), Feline (83%).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PDGF-B Antibody
Immunocytochemistry/ Immunofluorescence: PDGF-B Antibody [NBP1-52533]
Immunocytochemistry/Immunofluorescence: PDGF-B Antibody [NBP1-52533] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533]
Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533] - Analysis of anti-PDGFB antibody with human pancreas at concentration 3.75 ug/ml.Flow Cytometry: PDGF-B Antibody [NBP1-52533]
Flow Cytometry: PDGF-B Antibody [NBP1-52533] - Flow cytometric analysis of fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for PDGF-B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry-Paraffin
3.75 ug/ml
Peptide ELISA
1:128000
Application Notes
.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
TBS (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PDGF-B
Long Name
Platelet-derived Growth Factor B
Alternate Names
C-Sis, IBGC5, PDGF-2, PDGF2, PDGFB, SIS, SSV
Entrez Gene IDs
5155 (Human)
Gene Symbol
PDGFB
UniProt
Additional PDGF-B Products
Product Documents for PDGF-B Antibody
Product Specific Notices for PDGF-B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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