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PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73305

Novus Biologicals, part of Bio-Techne
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NBP2-73305
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 19-247 of human PDGFD(NP_079484) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free

Western Blot: PDGF-D/SCDGFB Antibody (OTI1C1)Azide and BSA Free [NBP2-73305]

Western Blot: PDGF-D/SCDGFB Antibody (OTI1C1)Azide and BSA Free [NBP2-73305]

Western Blot: PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free [NBP2-73305] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCDGFB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCDGFB.
Flow Cytometry: PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free [NBP2-73305]

Flow Cytometry: PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free [NBP2-73305]

Flow Cytometry: PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free [NBP2-73305] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SCDGFB antibody, and then analyzed by flow cytometry.

Applications for PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDGF-D

SCDGFB is encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq]

Long Name

Platelet-derived Growth Factor D

Alternate Names

PDGFD

Gene Symbol

PDGFD

Additional PDGF-D Products

Product Documents for PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDGF-D/SCDGFB Antibody (OTI1C1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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