PDGFR alpha Antibody (8E12F2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52528

Novus Biologicals, part of Bio-Techne
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NBP2-52528
NBP2-52528-0.025mg
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Key Product Details

Species Reactivity

Human, Feline

Applications

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8E12F2

Format

BSA Free

Concentration

1 mg/ml

View all PDGF R alpha Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PDGFR alpha (AA: 361-528) expressed in E. Coli.

Reactivity Notes

Feline reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

122.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDGFR alpha Antibody (8E12F2) - BSA Free

Western Blot: PDGFR alpha Antibody (8E12F2)BSA Free [NBP2-52528]

Western Blot: PDGFR alpha Antibody (8E12F2)BSA Free [NBP2-52528]

Western Blot: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis using PDGFRA mAb against HEK293 (1) and PDGFRA (AA: 361-528)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (8E12F2) [NBP2-52528] - PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Imaging of Optice Nerve. Primary Antibody (red, dilution1:800) and DAPI (blue). This image was submitted via customer Review.
Flow (Intracellular): PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Flow (Intracellular): PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Flow (Intracellular): PDGF R alpha Antibody (8E12F2) [NBP2-52528] - An intracellular stain was performed on HeLa cells with PDGF R alpha Antibody (8E12F2) NBP2-52528AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Applications for PDGFR alpha Antibody (8E12F2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 2 reviews rated 3 using NBP2-52528 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDGF R alpha

Platelet-derived growth factor receptors exhibit tyrosine-protein kinase activity and have been implicated in the control of cell proliferation, survival and migration. PDGF receptors, PDGFR-alpha and PDGFR-beta, have 5 extracellular immunoglobulin-like domains and an intracellular tyrosine kinase domain. Upon binding a PDGF, the receptors form homo-and heterodimers. Dimerization of the receptors results in phosphorylation in the complex. More than 10 different SH2-domain-containing molecules have been shown to bind to different autophosphorylation sites in the PDGF-alpha and beta receptors. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Gene Symbol

PDGFRA

Additional PDGF R alpha Products

Product Documents for PDGFR alpha Antibody (8E12F2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDGFR alpha Antibody (8E12F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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