PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-61020

Novus Biologicals, part of Bio-Techne
Discontinued Product
NB110-61020 has been discontinued. View all PDGF R alpha products.

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # RM0004-3G28

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PDGF R alpha Antibodies »

Product Specifications

Immunogen

Mouse PDGFR alpha (extracellular domain)

Reactivity Notes

Not yet tested in other species.

Specificity

This antibody recognizes mouse PDGF receptor alpha in western blot. No cross reactivity to mouse PDGF receptor beta.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free

Immunohistochemistry-Paraffin: PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free [NB110-61020]

Immunohistochemistry-Paraffin: PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free [NB110-61020]

Immunohistochemistry-Paraffin: PDGF Receptor alpha Antibody (RM0004-3G28) [NB110-61020] - PDGFR-a immunohistochemistry staining of paraffin sections of mouse pancreas from LPS exposed animals.

Applications for PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:400-1:800
Application Notes
For immunohistochemsitry, it is critical to use a mouse IgG preabsorbed secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDGF R alpha

Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Entrez Gene IDs

18595 (Mouse)

Gene Symbol

PDGFRA

UniProt

P26618

Additional PDGF R alpha Products

Product Documents for PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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