Pdi1p Antibody (38H8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05391

Novus Biologicals, part of Bio-Techne
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NBP1-05391
NBP1-05391-0.125ml
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Key Product Details

Species Reactivity

Yeast

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 38H8

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Pdi1p Antibodies »

Product Specifications

Immunogen

Yeast nuclear preparations

Reactivity Notes

S. cerevisiae

Localization

endoplasmic reticulum and nuclear envelope

Marker

Endoplasmic Reticulum Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

58.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pdi1p Antibody (38H8)

Western Blot: Pdi1p Antibody (38H8) [NBP1-05391]

Western Blot: Pdi1p Antibody (38H8) [NBP1-05391]

Western Blot: Pdi1p Antibody (38H8) [NBP1-05391] - Western Blot analysis of whole yeast protein extracts detected Pdi1p. Molecular weight detected is 58.2 kDa.

Applications for Pdi1p Antibody (38H8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Western Blot

1:10000
Application Notes
This Pdi1p (38H8) antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. In WB of yeast protein extracts a ~65 kDa band can be seen. In ICC/IF using yeast cells, endoplasmic reticulum staining can be seen.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Supplied as concentrated hybridoma cell culture media.

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pdi1p

One of the important aspects of secretory protein's folding process is the formation of native disulfide bonds which is catalyzed by enzyme protein disulfide isomerase (PDI) and yeast Saccharomyces cerevisiae has about 5 protein disulfide isomerase (PDI) family members (EUG1, MPD1, MPD2, EPS1 and PDI1/PDI1p). Pdi1p is a typical member of its family which is an essential gene and is considered homologus to human PDI with no significant differences; however, the two proteins are only 29% identical. It is a major ER protein with chaperon/co-chaperon activity and is involved in the retention of resident ER proteins as well as in recognizing proteins targeted for ER-associated degradation (ERAD). Yeast Pdi1p is a folding catalyst which introduces disulfide bonds into its substrates and rearranges or reduces pre-existing ones, and besides its oxidoreductase activity, Pdi1p acts as a molecular chaperone that also facilitates folding of proteins which do not contain disulfide bonds.

Alternate Names

MFP 1, MFP1, PDI, PDI 1, PDI1, Protein disulfide isomerase, Thioredoxin related glycoprotein 1, TRG 1, TRG1

Additional Pdi1p Products

Product Documents for Pdi1p Antibody (38H8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pdi1p Antibody (38H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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