PDIR Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92252
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VTFKSIVAFLKDPKGPPLWEEDPGAKDVVHLDSEKDFRRLLKKEEKPLLIMFYAPWCSMCKRMMPHFQKAATQLRGHAVLAGMNVYSSEFE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PDIR Antibody - BSA Free
![Western Blot: PDIR Antibody [NBP1-92252]](https://resources.bio-techne.com/images/products/PDIR-Antibody-Western-Blot-NBP1-92252-img0006.jpg "Western Blot: PDIR Antibody [NBP1-92252]")
Western Blot: PDIR Antibody [NBP1-92252]
Western Blot: PDIR Antibody [NBP1-92252] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
![Immunocytochemistry/ Immunofluorescence: PDIR Antibody [NBP1-92252]](https://resources.bio-techne.com/images/products/PDIR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92252-img0004.jpg "Immunocytochemistry/ Immunofluorescence: PDIR Antibody [NBP1-92252]")
Immunocytochemistry/ Immunofluorescence: PDIR Antibody [NBP1-92252]
Immunocytochemistry/Immunofluorescence: PDIR Antibody [NBP1-92252] - Staining of human cell line U-251 MG shows positivity in nucleoli, nucleus but not nucleoli and golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry: PDIR Antibody [NBP1-92252]
Immunohistochemistry: PDIR Antibody [NBP1-92252] - Immunohistochemistry analysis in human placenta and skeletal muscle tissues using NBP1-92252 antibody. Corresponding PDIA5 RNA-seq data are presented for the same tissues.
![PDIR Antibody Immunohistochemistry-Paraffin: PDIR Antibody Antibody [NBP1-92252]](https://resources.bio-techne.com/images/products/nbp1-92252_rabbit-polyclonal-pdir-antibody-104202412263519.jpg "Immunohistochemistry-Paraffin: PDIR Antibody Antibody [NBP1-92252]")
Applications for PDIR Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1.0 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PDIR
Alternate Names
FLJ30401, PDIREC 5.3.4.1, protein disulfide isomerase family A, member 5, protein disulfide isomerase-associated 5, Protein disulfide isomerase-related protein, protein disulfide-isomerase A5
Gene Symbol
PDIA5
Additional PDIR Products
Product Documents for PDIR Antibody - BSA Free
Product Specific Notices for PDIR Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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