PDK2 Antibody (7X6R7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16314
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7X6R7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 308-407 of human PDK2 (Q15119). YSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PDK2 Antibody (7X6R7)
Western Blot: PDK2 Antibody (7X6R7) [NBP3-16314]
Western Blot: PDK2 Antibody (7X6R7) [NBP3-16314] - Western blot analysis of extracts of various cell lines, using PDK2 Rabbit mAb (NBP3-16314) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.Immunohistochemistry-Paraffin: PDK2 Antibody (7X6R7) [NBP3-16314]
Immunohistochemistry-Paraffin: PDK2 Antibody (7X6R7) [NBP3-16314] - Immunohistochemistry of paraffin-embedded human colon using PDK2 Rabbit mAb (NBP3-16314) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for PDK2 Antibody (7X6R7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PDK2
Long Name
[Pyruvate dehydrogenase
Alternate Names
PDH kinase 2, PDHK2, PDKII
Gene Symbol
PDK2
Additional PDK2 Products
Product Documents for PDK2 Antibody (7X6R7)
Product Specific Notices for PDK2 Antibody (7X6R7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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