PDLIM4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1382

Novus Biologicals, part of Bio-Techne
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NB100-1382
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PDLIM4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DVVAVYPNAKVELV corresponding to C-Terminus according to NP_003678.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PDLIM4 Antibody

Western Blot: PDLIM4 Antibody [NB100-1382]

Western Blot: PDLIM4 Antibody [NB100-1382]

Western Blot: PDLIM4 Antibody [NB100-1382] - (1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NB100-1382]

Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NB100-1382]

Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NB100-1382] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NB100-1382]

Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NB100-1382]

Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NB100-1382] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for PDLIM4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1.0 -3.0 ug/ml
Application Notes
Western blot: Approx 37kDa band observed in lysates of cell line A431 (calculated MW of 35.4kDa according to NP_003678.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDLIM4

RIL was originally identified as a gene downregulated in H-ras-transformed cells. RIL was mostly expressed in nonmuscle epithelial cells with a pattern distinct from that of CLP-36. RIL protein was found to localize to actin stress fibers in nonmuscle cells similarly to CLP-36. However, RIL expression led to partially abnormal actin filaments showing thick irregular stress fibers not seen with CLP-36. The RIL PDZ-LIM protein is a regulator of actin stress fiber turnover.

Alternate Names

LIM domain protein, LIM protein RIL, PDZ and LIM domain 4, PDZ and LIM domain protein 4, Reversion-induced LIM protein, RILenigma homolog

Entrez Gene IDs

8572 (Human); 30794 (Mouse); 24915 (Rat)

Gene Symbol

PDLIM4

UniProt

NP_003678 (Human)

Additional PDLIM4 Products

Product Documents for PDLIM4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDLIM4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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