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PDLIM4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80833

Novus Biologicals, part of Bio-Techne
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NBP1-80833

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDLIM4 Antibody

Western Blot: PDLIM4 Antibody [NBP1-80833]

Western Blot: PDLIM4 Antibody [NBP1-80833]

Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 induces demethylation and re-expression of tumor suppressor genes. Cells were treated with SH-I-14 for 48 hr and subjected to western blot analysis. The lysates from the cells treated with SH-I-14 for 48 hr were analyzed by western blot with indicated antibodies. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/fulltext/4054), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NBP1-80833]

Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NBP1-80833]

Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP1-80833] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833]

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833]

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.

Applications for PDLIM4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 29137356). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDLIM4

PDLIM4, also known as PDZ and LIM domain protein 4, has 2 isoforms, a 330 amino acid long isoform that is 35 kDa and a short 246 amino acid isoform that is 26 kDa and found in brain. May be involved in bone development. Disease research is being performed on this protein involvement in osteoporosis, bronchiolitis, prostate cancer, prostatitis, multiple personality disorder, and asperger syndrome. The protein interacts with RBPMS, PTPN13, TRIP6, MYC, ZNF408, ABCA1, NFE2, SLC2A4, SSBP, TCF3, and ZBTB9 proteins.

Alternate Names

LIM domain protein, LIM protein RIL, PDZ and LIM domain 4, PDZ and LIM domain protein 4, Reversion-induced LIM protein, RILenigma homolog

Entrez Gene IDs

8572 (Human)

Gene Symbol

PDLIM4

UniProt

Additional PDLIM4 Products

Product Documents for PDLIM4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDLIM4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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