PDX-1/IPF1 Antibody (OTI2A12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47910

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A12

Concentration

1 mg/ml

View all PDX-1/IPF1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDX1 (NP_000200) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens pancreatic and duodenal homeobox 1 (PDX1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDX-1/IPF1 Antibody (OTI2A12)

Western Blot: PDX-1/IPF1 Antibody (OTI2A12) [NBP1-47910]

Western Blot: PDX-1/IPF1 Antibody (OTI2A12) [NBP1-47910]

Western Blot: PDX-1/IPF1 Antibody (2A12) [NBP1-47910] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1.
Immunocytochemistry/ Immunofluorescence: PDX-1/IPF1 Antibody (OTI2A12) [NBP1-47910]

Immunocytochemistry/ Immunofluorescence: PDX-1/IPF1 Antibody (OTI2A12) [NBP1-47910]

Immunocytochemistry/Immunofluorescence: PDX-1/IPF1 Antibody (2A12) [NBP1-47910] - Staining of HELA cells transiently transfected by pCMV6-ENTRY PDX1.
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (OTI2A12) [NBP1-47910]

Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (OTI2A12) [NBP1-47910]

Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (2A12) [NBP1-47910] - Staining of paraffin-embedded Human pancreas tissue using anti-PDX1 mouse monoclonal antibody.
View 2 more images

Applications for PDX-1/IPF1 Antibody (OTI2A12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:20000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDX-1/IPF1

Pdx1 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. It is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in PDX1 gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4)

Long Name

Pancreas/Duodenum Homeobox-1

Alternate Names

IDX1, IPF1

Entrez Gene IDs

3651 (Human)

Gene Symbol

PDX1

UniProt

P52945

Additional PDX-1/IPF1 Products

Product Documents for PDX-1/IPF1 Antibody (OTI2A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDX-1/IPF1 Antibody (OTI2A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.