PDX-1/IPF1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22150

Novus Biologicals, part of Bio-Techne
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NBP2-22150
NBP2-22150SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Mouse, Canine

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PDX-1/IPF1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human PDX1 protein (between residues 100-200) [UniProt P52945]

Reactivity Notes

Predicted to react with rat based on 100% sequence homology. Canine reactivity reported in scientific literature (PMID: 30332726).

Localization

Nucleus, cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDX-1/IPF1 Antibody - BSA Free

Western Blot: PDX-1/IPF1 AntibodyBSA Free [NBP2-22150]

Western Blot: PDX-1/IPF1 AntibodyBSA Free [NBP2-22150]

Western Blot: PDX1 Antibody [NBP2-22150] - WB analysis of PDX1 in INS1 cell lysate.
Immunocytochemistry/ Immunofluorescence: PDX-1/IPF1 Antibody - BSA Free [NBP2-22150]

Immunocytochemistry/ Immunofluorescence: PDX-1/IPF1 Antibody - BSA Free [NBP2-22150]

Immunocytochemistry/Immunofluorescence: PDX1 Antibody [NBP2-22150] - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: PDX-1/IPF1 Antibody - BSA Free [NBP2-22150]

Immunohistochemistry: PDX-1/IPF1 Antibody - BSA Free [NBP2-22150]

Immunohistochemistry: PDX1 Antibody [NBP2-22150] - IHC analysis of PDX1 in mouse pancreas.

Applications for PDX-1/IPF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200 - 1:500

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1.0 ug/ml
Application Notes
In Western blot a band is observed ~40 kDa, and in ICC/IF diffuse nuclear and cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDX-1/IPF1

PDX1, also known as IDX-1, IPF-1, STF-1, and MODY4 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. During development, PDX1 specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, it is required for maintaining the hormone-producing phenotype of the beta-cell. PDX1 is located in both the nucleus and cytosol on a cellular level, while in tissue it is distinct to the duodenum and pancreas. Defects in PDX1 gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). Recently, research of PDX1 ranges from an important role in the malaria parasite (PMID: 23039077) to mutations in PDX1 leading to neonatal diabetes (PMID: 20009086).

Long Name

Pancreas/Duodenum Homeobox-1

Alternate Names

IDX1, IPF1

Entrez Gene IDs

3651 (Human); 18609 (Mouse)

Gene Symbol

PDX1

UniProt

P52946, P52945

Additional PDX-1/IPF1 Products

Product Documents for PDX-1/IPF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDX-1/IPF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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