PEDFR/PNPLA2/ATGL Antibody (1H6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26374
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1H6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PEDFR/PNPLA2/ATGL Antibody (1H6)
Immunogen
A synthesized peptide derived from Human PEDFR/PNPLA2/ATGL [UniProt Q96AD5]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PEDFR/PNPLA2/ATGL Antibody (1H6)
Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (1H6) [NBP3-26374] -
Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (1H6) [NBP3-26374] - Overlay Peak curve showing HepG2 cells stained with PEDFR/PNPLA2/ATGL Antibody (1H6) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for PEDFR/PNPLA2/ATGL Antibody (1H6)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PEDFR/PNPLA2
Defects in PNPLA2 can lead to neutral lipid storage disease with myopathy (NLSDM), a neutral lipid storage disorder with myopathy but without ichthyosis.
ATGL antibodies can serve as useful tools for adipocyte studies and research on triglyceride processing and fat storage.
Long Name
Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2
Alternate Names
ATGL, Desnutrin, IPLA2-zeta, PEDF R, PNPLA2, TTS2
Gene Symbol
PNPLA2
Additional PEDFR/PNPLA2 Products
Product Documents for PEDFR/PNPLA2/ATGL Antibody (1H6)
Product Specific Notices for PEDFR/PNPLA2/ATGL Antibody (1H6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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