PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59390

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # AT18E6

Format

BSA Free

Concentration

1 mg/ml

View all PEDFR/PNPLA2 Antibodies »

Product Specifications

Immunogen

Recombinant human PEDFR/PNPLA2/ATGL (30-504aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free

Western Blot: PEDFR/PNPLA2/ATGL Antibody (AT18E6)BSA Free [NBP2-59390]

Western Blot: PEDFR/PNPLA2/ATGL Antibody (AT18E6)BSA Free [NBP2-59390]

Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste
Immunocytochemistry/ Immunofluorescence: PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free [NBP2-59390]

Immunocytochemistry/ Immunofluorescence: PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free [NBP2-59390]

Immunocytochemistry/Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free [NBP2-59390]

Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free [NBP2-59390]

Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Applications for PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PEDFR/PNPLA2

Patatin-like phospholipase domain-containing protein 2 (PNPLA2), also known as adipose triglyceride lipase (ATGL), is a lipolytic enzyme required for mobilization of fatty acids from triglyceride stores in adipose tissue. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. However, ATGL has now been shown to catalyze the initial step of triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Thus, ATGL and HSL coordinate to catabolize stored triglycerides in adipose tissue of mammals.

Defects in PNPLA2 can lead to neutral lipid storage disease with myopathy (NLSDM), a neutral lipid storage disorder with myopathy but without ichthyosis.

ATGL antibodies can serve as useful tools for adipocyte studies and research on triglyceride processing and fat storage.

Long Name

Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2

Alternate Names

ATGL, Desnutrin, IPLA2-zeta, PEDF R, PNPLA2, TTS2

Gene Symbol

PNPLA2

Additional PEDFR/PNPLA2 Products

Product Documents for PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PEDFR/PNPLA2/ATGL Antibody (AT18E6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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