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Peflin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31613

Novus Biologicals, part of Bio-Techne
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NBP1-31613

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Peflin. The exact sequence is proprietary.

Localization

Cytoplasm, Membrane, Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Peflin Antibody

Western Blot: Peflin Antibody [NBP1-31613]

Western Blot: Peflin Antibody [NBP1-31613]

Western Blot: Peflin Antibody [NBP1-31613] - Sample(30 ug whole cell lysate)A:293T 12% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Peflin Antibody [NBP1-31613]

Immunocytochemistry/ Immunofluorescence: Peflin Antibody [NBP1-31613]

Immunocytochemistry/Immunofluorescence: Peflin Antibody [NBP1-31613] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Peflin Antibody [NBP1-31613]

Immunohistochemistry-Paraffin: Peflin Antibody [NBP1-31613]

Immunohistochemistry-Paraffin: Peflin Antibody [NBP1-31613] - Paraffin-embedded SCC4 xenograft, using antibody at 1:100 dilution.

Applications for Peflin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Peflin

PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpainsmall subunit (CAPNS1; MIM 114170), sorcin (SRI; MIM 182520), grancalcin (GCA; MIM 607030), and ALG2 (PDCD6; MIM601057) (Kitaura et al., 2001 (PubMed 11278427)).(supplied by OMIM)

Alternate Names

ABP32, PEF protein with a long N-terminal hydrophobic domain, PEF1A, peflin, penta-EF hand domain containing 1, Penta-EF hand domain-containing protein 1, penta-EF-hand domain containing 1

Gene Symbol

PEF1

Additional Peflin Products

Product Documents for Peflin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Peflin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.