PEG3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47624

Novus Biologicals, part of Bio-Techne
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NBP2-47624
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PEG3 Antibodies »

Product Summary for PEG3 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRPRGMLYECQECGECFAHSSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCKDFRQFFATSEDLNTNQKIYDQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PEG3 Antibody

Western Blot: PEG3 Antibody [NBP2-47624]

Western Blot: PEG3 Antibody [NBP2-47624]

Western Blot: PEG3 Antibody [NBP2-47624] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line HepG2.
Immunocytochemistry/ Immunofluorescence: PEG3 Antibody [NBP2-47624]

Immunocytochemistry/ Immunofluorescence: PEG3 Antibody [NBP2-47624]

Immunocytochemistry/Immunofluorescence: PEG3 Antibody [NBP2-47624] - Staining human cell line Hep G2 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PEG3 Antibody [NBP2-47624]

Immunohistochemistry-Paraffin: PEG3 Antibody [NBP2-47624]

Immunohistochemistry-Paraffin: PEG3 Antibody [NBP2-47624] - Staining of human placenta shows strong nuclear positivity in trophoblastic in trophoblastic cells and decidua cells.
View 1 more images

Applications for PEG3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PEG3

In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. (provided by RefSeq)

Alternate Names

DKFZp781A095, KIAA0287PW1, paternally expressed 3, paternally expressed gene 3, Zinc finger and SCAN domain-containing protein 24, ZNF904, ZSCAN24Kruppel-type zinc finger protein

Entrez Gene IDs

5178 (Human)

Gene Symbol

PEG3

UniProt

Q9GZU2 (Human)

Additional PEG3 Products

Product Documents for PEG3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PEG3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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