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PEPD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86072

Novus Biologicals, part of Bio-Techne
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NBP1-86072

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PEPD Antibody

Immunohistochemistry-Paraffin: PEPD Antibody [NBP1-86072]

Immunohistochemistry-Paraffin: PEPD Antibody [NBP1-86072]

Immunohistochemistry-Paraffin: PEPD Antibody [NBP1-86072] - Analysis in human kidney and skeletal muscle tissues. Corresponding PEPD RNA-seq data are presented for the same tissues.
Western Blot: PEPD Antibody [NBP1-86072]

Western Blot: PEPD Antibody [NBP1-86072]

Western Blot: PEPD Antibody [NBP1-86072] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: PEPD Antibody [NBP1-86072]

Immunocytochemistry/ Immunofluorescence: PEPD Antibody [NBP1-86072]

Immunocytochemistry/Immunofluorescence: PEPD Antibody [NBP1-86072] - Staining of human cell line A549 shows localization to nucleoplasm.

Applications for PEPD Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PEPD

The PEPD gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rat

Alternate Names

aminoacyl-L-proline hydrolase, EC 3.4.13.9, Imidodipeptidase, MGC10905, peptidase Dimidodipeptidase, PRD, Prolidase, Proline dipeptidase, xaa-Pro dipeptidase, X-Pro dipeptidase

Gene Symbol

PEPD

Additional PEPD Products

Product Documents for PEPD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PEPD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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