Perforin Antibody (B-D48)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14582

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B-D48

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Perforin Antibodies »

Product Summary for Perforin Antibody (B-D48)

Immunogen

Natural human Perforin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Stable at 4C for 12 months.

Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells

Scientific Data Images for Perforin Antibody (B-D48)

Flow Cytometry: Perforin Antibody (B-D48) [NBP3-14582]

Flow Cytometry: Perforin Antibody (B-D48) [NBP3-14582]

Flow Cytometry: Perforin Antibody (B-D48) [NBP3-14582] - Flow cytometric analysis of perforin in human YT-Indy cell line with the Perforin monoclonal antibody.

Applications for Perforin Antibody (B-D48)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use 10 ul to label 10^6 cells or 100 ul of whole blood
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 1% BSA.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perforin

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Gene Symbol

PRF1

Additional Perforin Products

Product Documents for Perforin Antibody (B-D48)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Perforin Antibody (B-D48)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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